Fig. 8.

Ber-lipo inhibited the activation of TLR4/MyD88/NF-κB signaling pathway. (A) The binding activity of bergapten to TLR4, MyD88, IκBα, and NF-κB p65 proteins showed by an in silico molecular docking analysis. (B) The protein expressions of key members in the TLR4/MyD88/NF-κB pathway by Western blot, including TLR4, MyD88, the phosphorylated (p-p65) and basal NF-κB p65, p-IκBα, and IκBα. (C) The normalized expression of the proteins calculated by ImageJ software. (D) NF-κB p65 nuclear translocation by immunofluorescent staining and imaged by 40 × oil immersion lens of confocal microscope. Scale bar, 20 μm ∗P < 0.05 vs Control. #P < 0.05 vs Model. †P < 0.05.