Figure 2.

Both phasic contractions from Kit-ChR2(+) and (–) mice are blocked by anoctamin 1 specific antagonist. Channelrhodopsin-2 (ChR2) regulated the low-frequency and high-amplitude (LFHA) contractions of colonic muscle strips via interstitial cells of Cajal (ICC). (A) The effect of anoctamin 1 antagonists, T16Ainh-A01 (10-20 µM), on phasic contractions Kit-ChR2(+) mice. The light evoked LFHA contractions could not be reproduced under T16Ainh-A01 (10 µM). (B) The effect of T16Ainh-A01 on phasic contractions Kit ChR2(–) mice. The blue rectangles represented the light stimuli, and the red lines represented the administration of T16Ainh-A01. Note: here we point out the component contributed from ICC (yellow box), smooth muscle (red box), and neuronal (green box). (C) The effect of T16Ainh-A01 (10 µM) on the mean integrated isometric force. *P < 0.001. Note: the phasic contractions either from Kit-ChR2(+) or (–) mice are both blocked by T16Ainh-A01 (10-20 µM), although some activity remained in a population of mice, indicating both of phasic contractions are from ICC.³