Fig. 3. (a) Composite FLIM image series of a single glioma cell after hypericin incubation, PI supplementation, and resorufin conversion. (b) Evolution of the overall fluorescence intensities of hypericin, resorufin and PI recorded in each image of the series. The fluorescence spectra acquired at the position markers are displayed in Fig. 4. The acquisition parameters of the imaging series are displayed above.
