Table 4.

Identification of antioxidant and antimicrobial compounds obtained from Bacillus LB7 by the mass spectrometer analysis, based on the Metabolic in silico Network Expansions database.

Strain	Band	m/z	Identification (MINE)	MINE code	Nature	Associated function
LB7 grown with 0.01 M Glucose	A	232.05	Phosphinate by-product	566,860	–	Antifungal, herbicidal, antiparasitic, antimicrobial
	B	275.2	Myxalamide C by-product	141,593	–	Antibacterial
LB7 grown at pH 12	A	265	Dihydroxymandelic Acid by-product	94,678	Phenol	Antioxidant-metabolite of norepinephrine
LB7 grown with 1,5 M NaCl	A	226.1	Amide biotine by-product	261,939	Carboxamide	Antioxidant, antibacterial
	A	232.05	Phosphinate by-product	566,860	Acid Anhydride acyclic phosphorus	Antifungal, herbicidal, antiparasitic, antimicrobial
	A	240.1	Flavone by-product	231,654	Flavonoid	Antioxidant, anti-inflammatory, anti-mutagenesis
	A	232.05	Phosphinate by-product	566,860	Acid Anhydride acyclic phosphorus	Antifungal, herbicidal, antiparasitic, antimicrobial
LB7 grown at 37°C	A	240.1	Flavone by-product	231,654	Flavonoid	Antioxidant, anti-inflammatory anti-mutagenesis

Each strain has a different profile of identified metabolites, including antioxidants such as 15,9′-dicis-phytofluene by-product and antimicrobials including Aurachin by-product and Neomethymicin or 10-Deoxymetinolide by product (Table 5). The LB8 strain produces siderophores and molecules associated with radiation response, critical for survival in their natural environment in a high-altitude hydrothermal system in the Altiplano (Supplementary Table S3). MINE code corresponds to the KEGG codification.