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. 2024 Sep 30;15:1463854. doi: 10.3389/fmicb.2024.1463854

Figure 1.

Figure 1

Organization of the isc and suf operons in selected bacteria. (A) Alphaproteobacteria such as C. crescentus and R. sphaeroides possess a single operon containing iscRS followed by suf genes. In Gammaproteobacteria, P. aeruginosa has a single isc operon, while E. coli and Enterobacteria have two distinct operons, isc and suf. (B) Schematic drawing showing each gene, and the positions of the primer pairs designed to amplify some of the genes junctions. RT-PCR using each primer pair with C. crescentus NA1000 total genomic DNA (DNA), cDNA from total RNA (cDNA) or total RNA (RNA). Marker, 1 kb Plus DNA marker. The sizes of the nearest bands are indicated for reference. Created in BioRender. Santos, N. (2024) BioRender.com/o98n394.