Figure 3.
Phenotypic analysis of the iscR mutant. (A) Growth of strains NA1000 (wt) containing the empty pMR20 plasmid (black line), ΔiscR containing the empty pMR20 plasmid (dark green line) and ΔiscR pMR20-iscR (blue line) was carried out in PYE and in PYE with the addition of 100 μM DP at 30°C with agitation. Growth was assessed by measuring the OD600 nm of six independent biological replicas. (B) The cultures of strains NA1000 (negative control), ΔiscR and Δfur (positive control) were grown in PYE up to midlog phase and incubated with 0 (control), 0.5 μg/mL or 1 μg/mL Streptonigrin for 24 h. The cultures were subjected to serial dilutions followed by plating on PYE and after 3 days at 30°C CFU counts were taken. These images are representative of 2 independent biological replicates.