Figure 5.
The intratumoral VSV-NDV treatment of B16 melanoma induces the enrichment and activation of innate immune cells. C57BL6/J mice were implanted with B16OVA cells subcutaneously on contralateral flanks. After seven days, the mice (N = 6–8) were injected intratumorally with VSV-NDV (VN) at a dose of 1 × 107 TCID50 or PBS in 50 µL on day 0, 3, 6. TDLNs or tumors were analyzed by flow cytometry for (A) for iNKT cell number and CD69 expression, (B) NK cell number, CD69 expression, IFN-γ expression, and NKG2D expression in the TDLNs and mature NK cells, NKG2D+ NK cells, and IFN-γ in the spleen, or (C) for DC number, CD86 expression, and MHC-I expression in TDLNs. Data points indicate values of individual replicates (and group means ± SD are presented as bars); * indicates p ˂ 0.05, ** p ˂ 0.01, *** p ˂ 0.001, **** p ˂ 0.0001.