FIG. 10.

Association of extracellular virus with actin tails. HeLa cells, which stably express GFP-actin (green), were infected with wild-type vaccinia virus. Unpermeabilized cells were stained with MAb 19C2 to the B5R membrane protein followed by Cy5-conjugated donkey anti-rat antibody (red) and Hoechst dye to stain DNA (blue). Entire cells were imaged by confocal microscopy as a series of optical sections. Starting from the bottom of one cell, every four sequential sections (1 to 4, 5 to 8, 9 to 12, and 13 to 16) were summed, and the set of four maximum-intensity projections is shown. The extracellular virions appear red at the tips of green actin tails and yellow if overlying an actin tail.