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Necroptosis markers correlate with the level of lactate. Western blot analysis of the necroptosis marker pMLKL (A). Viability through the fed‐batch culture (B). Quantification of the level of pMLKL (C), error bars represent ± SD of two technical replicates, western blot signals on each day were normalised to the day 6 value. Lactate levels measured in the media (D). Whole cell lysate was separated using SDS‐PAGE gel electrophoresis.
