Table 1.
Ingredients and analyzed proximate composition of the experimental diets supplemented with different levels of vitamin D3 and vitamin K3.
| Ingredients (%) | (0.07/0.01) | (0.20/0.58) | (0.19/1.65) | (0.51/0.74) | (0.56/1.00) |
|---|---|---|---|---|---|
| Fishmeal | 10 | 10 | 10 | 10 | 10 |
| Defatted squid meal | 1 | 1 | 1 | 1 | 1 |
| Squid meal | 2 | 2 | 2 | 2 | 2 |
| Casein | 7.5 | 7.5 | 7.5 | 7.5 | 7.5 |
| Soy protein concentrate | 16 | 16 | 16 | 16 | 16 |
| Wheat gluten meal | 12.9 | 12.9 | 12.9 | 12.9 | 12.9 |
| Corn gluten meal | 12.9 | 12.9 | 12.9 | 12.9 | 12.9 |
| Soybean meal | 6.5 | 6.5 | 6.5 | 6.5 | 6.5 |
| Wheat, whole | 9 | 9 | 9 | 9 | 9 |
| Rapeseed oil | 3.6 | 3.6 | 3.6 | 3.6 | 3.6 |
| Fish oil | 6 | 6 | 6 | 6 | 6 |
| Linseed oil | 3.6 | 3.6 | 3.6 | 3.6 | 3.6 |
| Soybean lecithin | 2 | 2 | 2 | 2 | 2 |
| L-Lysine | 0.97 | 0.97 | 0.97 | 0.97 | 0.97 |
| L-Methionine | 0.27 | 0.27 | 0.27 | 0.27 | 0.27 |
| L-Threonine | 0.16 | 0.16 | 0.16 | 0.16 | 0.16 |
| CaHPO4.2H2O | 1 | 1 | 1 | 1 | 1 |
| Carboxymethyl cellulose | 0.6 | 0.6 | 0.6 | 0.6 | 0.6 |
| Mineral premix * | 2 | 2 | 2 | 2 | 2 |
| Vitamin premix ** | 2 | 2 | 2 | 2 | 2 |
| Vitamin D3 (mg/kg) | 0 | 0.04 | 0.04 | 0.5 | 0.5 |
| Vitamin K3 (mg/kg) | 0 | 6 | 12 | 6 | 12 |
| Diet proximate composition (%) | |||||
| Moisture | 6.42 ± 0.13 | 6.28 ± 0.03 | 6.29 ± 0.04 | 6.41 ± 0.08 | 7.40 ± 0.05 |
| Crude protein | 44.81 ± 0.14 | 44.32 ± 0.37 | 44.60 ± 0.36 | 44.51 ± 0.02 | 44.34 ± 0.07 |
| Crude lipid | 21.64 ± 0.59 | 23.33 ± 0.46 | 22.74 ± 0.16 | 23.03 ± 1.15 | 21.57 ± 0.33 |
| Ash | 6.46 ± 0.08 | 6.20 ± 0.01 | 5.96 ± 0.24 | 6.10 ± 0.09 | 6.04 ± 0.02 |
| Analyzed dietary vitamins | |||||
| Vitamin D3 (mg/kg) | 0.07 ± 0.03 | 0.20 ± 0.04 | 0.19 ± 0.04 | 0.51 ± 0.10 | 0.56 ± 0.05 |
| Vitamin K3 (mg/kg) | 0.01 ± 0.00 | 0.58 ± 0.04 | 1.65 ± 0.05 | 0.74 ± 0.04 | 1.00 ± 0.06 |
| Supplemented dietary vitamins | |||||
| Vitamin D3 (mg/kg) | 0 | 0.04 | 0.04 | 0.5 | 0.5 |
| Vitamin K3 (mg/kg) | 0 | 6 | 12 | 6 | 12 |
* Supplied the following mineral (g/kg mix): calcium hydrogen phosphate dihydrate ((H2PO)2Ca)—1.605, calcium carbonate (CaCO3)—4, Iron(II) sulfate heptahydrate (FeSO47H2O)—1.5, magnesium sulfate heptahydrate (MgSO47H2O)—1.605, dipotassium hydrogen phosphate (K2HPO4)—2.8, disodium hydrogen phosphate hydrate (Na2PO4H2O)—1, aluminium sulfate hexahydrate (Al(SO4)36H2O)—0.02, zinc sulfate pentahydrate (ZnSO45H2O)—0.12, copper(II) sulfate pentahydrate (CuSO45H2O)—0.12, potassium iodide (KI)—0.02, manganese(II) sulfate monohydrate (MnSO4H2O)—0.08, cobalt(II) sulfate heptahydrate (CoSO47H2O)—0.08, and selenium (Se)—0.0003. ** Supplied the following vitamin (g/kg mix): vitamin B1 (thiamine)—0.06, vitamin B2 (riboflavin)—0.08, vitamin B6 (pyridoxine)—0.04, vitamin B5 (calcium pantothenate)—0.1169, vitamin B3 (nicotinic acid)—0.2, biotin (vitamin H)—0.001, vitamin B9 (Folic Acid)—0.04, vitamin B12 (cyanocobalamin)—0.0009, choline—2.7, myo-inositol—2, vitamin C (ascorbic acid)—5, vitamin E (α-tocopherol)—0.25, vitamin A (retinol acetate)—0.025, and ethoxyquin—0.1.