Table 2.
Genes and primer sequences used in the gene expression.
| Gene | Forward Primer | Reverse Primer | Annealing Temperature Tm |
Accession Number |
|---|---|---|---|---|
| House Keeping Gene | ||||
|
β-actin2 (Beta actin 2) |
TCTGTCTGGATCGGAGGCTC | AAGCATTTGCGGTGGACG | 58.1 | X89920 |
|
ef1α (Elongation factor 1 alpha) |
CTTCAACGCTCAGGTCATCAT | GCACAGCGAAACGACCAAGGGGA | 60 | AF184170 |
|
rpl27 (Ribosomal protein L27) |
AAGAGGAACACAACTCACTGCCCCAC | GCTTGCCTTTGCCCAGAACTTTGTAG | 68 | AY188520 |
| Bone Biomarker | ||||
|
bmp2 (Bone morphogenetic protein 2) |
GTGGCTTCCATCGTATCAACATTTT | GCTCCCCGCCATGAGT | 60 | JF261172.1 |
|
runx2 (Runt-related transcription factor 2) |
GCCTGTCGCCTTTAAGGTGGTTGC | TCGTCGTTGCCCGCCATAGCTG | 61 | AJ619023 |
| (osx)
Osterix |
CAGTCAGGGATTCAGCAACA | GGTGAAGGAGCCAGTGTAGG | 60 | ERR22591_isotig06993 |
|
alp (Alkaline phosphatase) |
AGAACG CCCTGACGC TGCAA | TTCAGTATACGAGCA GCCGTCAC | 61 | AY266359 |
|
opn (Osteopontin) |
AAGATGGCCTACGACATGACAGAC | CCTGAAGAGCCTTGTACACCTGC | 61 | AY651247 |
|
on (Osteonectin) |
AAAATGATCGAGCCCTGCATGGAC | TACAGAGTCACCAGGACGTT | 61 | AY239014 |
|
oc (osteocalcin) |
AGCCCAAAGCACGTAAGCAAGCTA | TTTCATCACGCTACTCTACGGGTT | 58.1 | AF048703 |
| Calcium Regulating Gene | ||||
|
vdrβ (Vitamin D receptor β) |
TGACGACTCCTACTCTGACT | CTGCTGCCCTGCTCTTGGTA | 62.3 | XM_030420365.1 |
|
cyp27a1 (Cytochrome P450 family 27 subfamily A member 1) |
TGGCTCTACAAGTTTGGCTTTGA | TGAACCGCAGCG TGTCTTT | 60 | AM885865 |
|
stc2 (Stanniocalcin 2) |
CTGGAGCAAGTAGTGGGAT | CCTGTAGCCCTCGTATCTCG | 62 | XM_030395344 |
|
casr (Calcium sensing receptor) |
GCTTCTCCAGCTCGCTCATC | AGGCGGGCTGGCGTAA | 60 | AJ289717 |
|
pthrp (Parathyroid hormone related protein) |
GAGGCAAATGAA TGGAACAG | TGGCCAGCTCAA AACTTGT | 60 | AF197904 |
|
pth1r (Parathyroid hormone 1 receptor) |
GAACCTGCCCGG CTACGTGAAG | GCTCCTGTCCCG ACGAGGGTAT | 60 | AJ619024 |
|
mgp (Matrix gla protein) |
CGCCCGAAATACACCTCAGA | GACGGACGGATACTAGGAGTCTA | 60 | AY065652 |
|
mmp9 (Matrix metallopeptidase 9) |
ATTCAGAAGGTGGAGGGAGCG | CATTGGGGACACCACCGAAGA | 60 | AM905938 |
|
ctsk (Cathepsin K) |
AGCGAGCAGAACCTGGTGGAC | GCAGAGTTGTAGTTGGGGTCGTAG | 60 | DQ875329 |
|
trap (Tartrate-resistant acid phosphatase) |
CTTAATCGTTGCCATCCCTGTG | CTCCCATCTGCTCTGCTACTTTG | 60 | FM147928 |