Table 8.
Relative gene expression of intestinal calcium regulators in gilthead seabream juveniles fed with different levels of dietary vitamin D3 and vitamin K3.
| Relative Gene Expression—Intestine | Two-Way ANOVA (p Value) |
|||||||
|---|---|---|---|---|---|---|---|---|
| Gene | (0.07/0.01) | (0.20/0.58) | (0.19/1.65) | (0.51/0.74) | (0.56/1.00) | VD | VK | VD × VK |
| casr | 0.43 ± 0.11 a | 1.96 ± 0.85 b | 3.75 ± 1.31 c | 2.01 ± 0.41 b | 0.62 ± 0.1 ab | 0.01 | 0.53 | 0.00 |
| pth1r | 0.34 ± 0.04 a | 1.83 ± 1.04 b | 3.42 ± 0.65 c | 1.98 ± 0.80 b | 0.87 ± 0.36 ab | 0.16 | 0.80 | 0.01 |
| pthrp | 0.46 ± 0.09 a | 2.07 ± 1.43 ab | 4.17 ± 1.13 b | 2.66 ± 1.48 ab | 0.62 ± 0.15 a | 0.30 | 0.26 | 0.01 |
| stc2 | 0.52 ± 0.25 a | 2.02 ± 1.22 ab | 3.71 ± 1.42 b | 3.28 ± 1.09 b | 0.76 ± 0.27 a | 0.44 | 0.23 | 0.00 |
| pxr | 0.50 ± 0.32 | 1.95 ± 0.69 | 2.30 ± 1.42 | 1.92 ± 0.20 | 1.97 ± 3.05 | 0.85 | 0.84 | 0.88 |
Different letters (a, b, c) in the row denote significant differences between groups fed different diets (mean ± SD, n = 3, p < 0.05). (number of fish sampled = 3 per tank).