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. 2023 Oct 15;19(1):2300323. doi: 10.1002/biot.202300323

FIGURE 1.

FIGURE 1

Illustration of CRISPR/Cas9 construct. (A) Schematic presentation of the three α‐1,3‐fucosyltransferase (NbFucT3‐5) genes targeted. Position of the double strand break induced by SpCas9/G1.3 complex is represented with a red triangle. Target nucleotide sequence (with protospacer adjacent motif underlined) is identical among the three genes and the matching spacer sequence of the guide G1.3. (B) Transformation vector pBG04 (T‐DNA region, not to scale): E‐35S P – enhanced CaMV 35S promoter; tRNA ‐ tRNAGly from Arabidopsis thaliana; osgRNA – optimized sgRNA scaffold with extended hairpin.[ 14 ]; 35S T, E9 T, NOS T: respective CaMV 35S, rbcS and nopaline synthase gene terminator; NPTII – neomycin phosphotransferase II; SpCas9 NLS Streptococcus pyogenes Cas9 with nuclear localization signals; LB and RB – T‐DNA left and right border.