Table 1.

Oral HPV sampling and detecting protocols by Scope Oral Rinse and LolliSponge.

Step	Description	Procedures
1	Sample collection	Scope Oral Rinse: (i) dispense 10 mL of Original Mint Scope mouthwash into a sterile 50 mL Falcon tube; (ii) rinse orally with the mouthwash for 60 s, carefully reaching all parts of the mouth, avoiding gargling; then, spitting back into the Falcon tube. LolliSponge: (i) open the LolliSponge test tube holding the device by the cap and insert it in the mouth; (ii) gently move it around the mouth and scrape into suspected lesions, for 60 s, so that the sponges are well moistened; (iii) place the cap on the LolliSponge test tube, screw it on and securely close the test tube.
2	Sample process	Scope Oral Rinse: (i) centrifuge at 1600 rpm for 10′ to isolate the cellular component; (ii) resuspended the pellet in 1 to 4 mL of phosphate-buffered saline (PBS) and centrifuged again at 13,000 rpm for 5 min; (iii) after removing the supernatant, store the samples at −20 °C or process immediately. LolliSponge: (i) centrifuge at 1800 rpm for 10′ to isolate saliva form the absorbent sponge by centrifugating the device at 1800 rpm for 1 min; (ii) after removing the supernatant, store the samples at −20 °C or process immediately.
3	HPV-DNA extraction	Scope Oral Rinse and LolliSponge Use QIAamp Mini Kit (Qiagen, Hilden, Germany) to extract DNA following manufacturers’ protocol.
4	HPV detection and genotype	Scope Oral Rinse and LolliSponge Use INNO-LiPA HPV Genotyping Extra II (Fujirebio, Tokyo, Japan) and Basic Local Alignment Search Tool (BLAST) to identify HPV genotypes. Perform Nested PCR, followed by Sanger sequencing and Basic Local Alignment Search Tool (BLAST) alignment, for samples positive to Inno-lipa HPV controls, but not genotyping by the diagnostic kit.