Table 1.
Composition of test strips used for the ICAs and the assay conditions.
| Parameter | ICA of Salmonella with AuNPs | ICA of Listeria with AuNPs | ICA of Salmonella with PBNPs | ICA of Listeria with PBNPs | Double ICA with PBNPs |
|---|---|---|---|---|---|
| Absorption pad | Yes | Yes | Yes | Yes | Yes |
| Sample pad | Yes | Yes | No | No | No |
| Conjugate pad | Yes | Yes | No | No | No |
| Configuration of the test strip | Full | Full | Shortened * | Shortened | Shortened |
| The immobilization buffer | PBS | PBS | PBS+ ** | PBS+ | PBS+ |
| MAb in the T zone(s), mg/mL |
1 | 1 (clone LZF7) |
2.5 | 2.5 (clone LZF7) |
2.5 for both MAb |
| GAMI in the C zone, mg/mL | 0.5 | 0.5 | 0.5 | 0.5 | 1 |
| Labeled MAb immobilized on the conjugate pad, OD520 | 6 | 6 (clone LZH1) | No | No | No |
| Labeled MAb added into the tested sample, µL | No | No | 1.5 | 1.5 (clone LZH1) | 1.5 (both) |
| The sequence of the zones on the test strips (top down) | C zone → T zone |
C zone → T zone |
C zone → T zone |
C zone → T zone |
C zone → T1 zone (Listeria detection) → T2 zone (Salmonella detection) |
* Test strips are cut to the bottom edge of the working membrane. ** PBS+ is PBS containing 0.1% sodium azide, 0.25% sucrose, and 0.25% BSA