Fig. 5.

Lifetime measurements of SthK_C-term-S361Acd-V416C with [Fe(phenM)₃]²⁺ and [Ru(bpy)₂phenM]²⁺. Chemical structures of acceptors and legends for all plots shown on top. (A-B) Representative Weber plots of phase delay and modulation ratio for SthK_C-term-S361Acd-V416C labeled with [Fe(phenM)₃]²⁺ (A) and [Ru(bpy)₂phenM]²⁺(B). Fits of the data using the single Gaussian model are shown with phase delay as solid curves and modulation ratio as dashed curves. (C-D) Spaghetti plots showing distance distributions from the model fits with apo (thin black curves, n=4), with 1.23 mM cAMP (thin red curves, n=4) and 1.23 mM cGMP (thin green curves, n=4) for [Fe(phenM)₃]²⁺ (C) and [Ru(bpy)₂phenM]²⁺(D). Distributions predicted by chiLife are overlayed in dashed curves. (E-F) Summary of Gaussian fit standard deviations, $\overline{r}$, versus average distances, $\sigma$, for [Fe(phenM)₃]²⁺ (E) and [Ru(bpy)₂phenM]²⁺ (F), with average values as cross marks.