Figure 4.

Inhibition of PGPC-induced expression of pro-inflammatory and atherogenic genes in RAW264.7 cells by Oxy210. RAW264.7 cells were treated in DMEM containing 0.1% FBS overnight and then pretreated for 2 h with Oxy210 (5 μM) in DMEM containing 0.1% FBS. The cells were then treated with PGPC (50 μM) in the absence or presence of Oxy210 (5 μM). After 4 (A) or 24 (B) hours, RNA was extracted and analyzed by Q-RT-PCR for the expression of the genes as indicated and normalized to Oaz1 expression. Data from a representative experiment are reported as the mean of triplicate determinations ± SD (## p < 0.01 vs. PCPG; ** p < 0.01 vs. control).