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. 2024 Sep 30;13(19):1632. doi: 10.3390/cells13191632

Figure 5.

Figure 5

Inhibition of TNF-α-induced expression of VCAM-1 in HAECs and THP-1 cells by Oxy210. (A) The HAECs were pretreated with Oxy210 (5 μM) in M199 containing 1% FBS overnight and then treated with TNF-α (50 ng/mL) in the absence or presence of Oxy210 (5 μM). After 8 h, the RNA was extracted and analyzed by Q-RT-PCR for the expression of VCAM-1 and normalized to GAPDH expression. Data from a representative experiment are reported as the mean of triplicate determinations ± SD (## p < 0.01 vs. TNF-α; ** p < 0.01 vs. control). (B) The THP-1 cells were pretreated with Oxy210 (5 μM) in RPMI containing 1% FBS overnight and then treated with TNF-α (50 ng/mL) in the absence or presence of Oxy210 (5 μM). After 24 h, the RNA was extracted and analyzed by Q-RT-PCR for the expression of VCAM-1 and normalized to GAPDH expression. Data from a representative experiment are reported as the mean of triplicate determinations ± SD (## p < 0.01 vs. TNF-α; ** p < 0.01 vs. control).