FIGURE 4.

Dh31/Dh44-induced changes in spike pattern and KCa channel conductance. (a) Quantification of spike frequency adaptation with ISIx/ISI₁ ratio for Dh31, Dh44, Dh31 and Dh44, Dh31/Dh44/apamin, and Dh31/Dh44/cadmium (N=5). (b-d) Quantification of spike irregularity of CV, LV, and CV2 for varying conditions with (+) and without (−) Dh31, Dh44, apamin, and cadmium (N=6 each). (e) Current traces of control, Dh31, Dh44, and Dh31/Dh44. (f) I-V relationship of KCa conductance and peak amplitude of K_Ca for control, Dh31, Dh44, and Dh31/Dh44 (n=4 each). (g) Quantification of Peak Amplitude of KCa for varying conditions with (+) and without (−) Dh31, Dh44, apamin, and cadmium (N=4 each). (h) Current traces of control and both Dh31 and Dh44 together. (i) Quantification of Peak Amplitude KCa for control and both Dh31 and Dh44 together. One-way ANOVA with multiple comparisons was used. A p-value < 0.05 was considered statistically significant, with asterisks indicating p-values as follows: *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.