FIGURE 6.

Synaptic potentials show complex patterns under Dh31/Dh44 influence and possible involvement of Dh31-positive DN1 clock neurons. (a) Probability density of PSP inter event intervals for control (N=3), Dh31 (N=3), Dh44 (N=30), Dh31/Dh44 (N=3), and Dh31/Dh44/apamin (N=4). (b) Time between each event, PSP inter-event interval, was quantified from conditions with (+) and without (−) Dh31, Dh44, and apamin. (c) The interval times of two adjacent PSPs were projected into two-dimensional space, and Gaussian Mixture Model (GMM) was used to computationally capture the geometry of their probability distribution. (d) The area under the curve (AUC) in receiver operating characteristic (ROC) analysis based on interval timing of PSPs in PI neurons. (e) Whole-mount brain (left) and thoracic ganglion (right) immunostaining of a R20A02-AD;R18H11-DBD>UAS-thGFP fly with anti-GFP (green) and anti-nc82 (magenta). Scale bar indicates 300 μm and 30 μm in inset. The statistics used were unpaired t-test with *p < 0.05 and **p < 0.01 and ns indicated non-significant.