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. 2024 Oct 9;13(19):1670. doi: 10.3390/cells13191670

Figure 3.

Figure 3

Identification of the p21 C region sequence in mediating Trapper binding. (A) Sub-fragments of the p21 C region, numbered from 1 to 7, were used for further analysis. (B) The fold increase in luciferase activity over the control, resulting from the transfection of the C fragment presented in panel (A), along with the Trapper construct, is shown (N = 3). (C) Similar to panel (B), but using the full-length PSMA3 instead of the Trapper construct (N = 3). (D) The RRLIF sequence within the C fragment is compared to that corresponding sequence in the N fragment. (E) In the context of the p21 131–164 aa C fragment, the RRLIF motif was mutated to RRLAF and subjected to the split luciferase assay with both Trapper and the PSMA3 (N = 3). (F) The expression levels of the plasmids used in panel (E) are shown, with Ponceau staining serving as a loading control. (G) The RRLIF box in the full-length p21 was mutated to RRLAF and analyzed with both Trapper and the PSMA3 (N = 3). (H) The expression level of the plasmids used in panel (G), with Ponceau staining as a loading control.