Fig. 2.

Typical chromatograms for the separation of NIC (10.0µg/mL, 2.05 min), CAF (20.0 µg /mL, 3.9 min) in pure form, dosage form (2.5µg/mL, 2.05 min), CAF (25.0 µg /mL, 3.9 min) and blank. Chromatographic system: shim-pack cyano column (150 mm × 4.6 mm with 5.0 µm particle size, pressure adjusted to 1960 psi, pressure adjusted to 1960 psi) Mobile phase mobile phase consisting of Glycerol: 0.2 M OPA (5: 95% v/v), pH 2.5 flow rate, 1.0 mL/min, UV detection at 260 nm; column temperature, 40 degrees Celsius