Figure 5.

Detection of transcription factors in neuronal cells RN33B (a) and SW10 (b) treated with VCR alone as well as with co-application of NIM. After the transfer of the proteins separated by SDS-PAGE onto nitrocellulose membranes, phosphorylation and total protein levels of the cell signaling components were determined by specific antibodies. The GAPDH protein level was used as a loading control. The Western blot shown is representative of the results from three independent biological replicates.