Table 1.
List of used antibodies.
| Antibody | Species | Dilution | Dilution Buffer | Manufacture |
|---|---|---|---|---|
| AKT (40D4) #2920 | Mouse IgG1 | 1:2000 | 5% MP in TBS-T | Cell Signaling Technology (Danvers, MA, USA) |
| Phospho-Akt (Ser473) (D9E) #4060 | Rabbit IgG | 1:1000 | 5% BSA in TBS-T | Cell Signaling Technology (Danvers, MA, USA) |
| CREB (48H2) #9197 | Rabbit IgG | 1:1000 | 5% BSA in TBS-T | Cell Signaling Technology (Danvers, MA, USA) |
| Phospho-CREB (Ser133) (87G3) #9198 | Rabbit IgG | 1:1000 | 5% MP in TBS-T | Cell Signaling Technology (Danvers, MA, USA) |
| ERK 1/2 (T202/ Y204) #9102 | Rabbit IgG | 1:1000 | 5% BSA in TBS- | Cell Signaling Technology (Danvers, MA, USA) |
| Phospho-ERK 1/2 (Thr202/Tyr204) #9101 | Rabbit IgG | 1:1000 | 5% BSA in TBS-T | Cell Signaling Technology (Danvers, MA, USA) |
| LMO4 (D6V4Z) #81428 | Rabbit IgG | 1:1000 | 5% BSA in TBS-T | Cell Signaling Technology (Danvers, MA, USA) |
| GAPDH (14C10) #2118 | Rabbit IgG | 1:1000 | 5% BSA in TBS-T | Abcam (Cambridge, UK) |
| STAT3 (124H6) #9139 | Mouse IgG2a | 1:1000 | 5% MP in TBS-T | Cell Signaling Technology (Danvers, MA, USA) |
| Phospho-STAT3 (Tyr705) (3E2) #9138 | Mouse IgG1 | 1:1000 | 5% MP in TBS-T | Cell Signaling Technology Inc. (Danvers, MA, USA) |
| Anti-Rabbit IgG, HRP-linked Antibody #7074 | Goat | 1:1000 | 2% MP in TBS-T | Cell Signaling Technology Inc. (Danvers, MA, USA) |
| Anti-Mouse IgG, HRP-linked Antibody #7076 | Horse | 1:1000 | 2% MP in TBS-T | Cell Signaling Technology Inc. (Danvers, MA, USA) |
Abbreviations: AKT, protein kinase B; BSA, bovine serum albumin; CREB, cAMP response element-binding protein; GAPDH, glyceraldehyde-3-phosphatedehydrogenase; ERK, extracellular signal-regulated kinases; HRP, horseradish peroxidase; IgG, immunoglobulin G; LMO4, LIM domain only 4; MP, milk powder; Stat, signal transducer and activator of transcription.