Table 1.
Corticosterone levels in the plasma and oxidative stress in the amygdala.
| Conditions | Water | AO | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Ctrl | IEI | UMS | IEI/UMS | Ctrl | IEI | UMS | IEI/UMS | ||
| CORT in plasma (ng/mL) | 90.34 ± 14.89 | 140.21 ± 9.56 a | 105.83 ± 11.09 | 147.23 ± 5.74 aa | 84.34 ± 12.11 | 108.81 ± 6.27 | 106.23 ± 9.77 | 127.72 ± 10.07 | |
| OS in Amygdala |
MDA | 5.24 ± 0.67 | 11.34 ± 1.81 a | 6.59 ± 0.95 | 12.34 ± 1.19 aac | 4.43 ± 0.80 | 8.74 ± 1.54 | 5.39 ± 0.60 | 7.34 ± 0.94 δ |
| NO | 73.07 ± 12.09 | 119.09 ± 9.74 a | 86.32 ± 7.59 | 125.89 ± 9.58 aa | 78.67 ± 13.03 | 101.09 ± 6.86 | 76.32 ± 7.59 | 109.69 ± 8.35 | |
| SOD | 67.09 ± 9.64 | 31.19 ± 6.67 a | 54.06 ± 5.58 | 21.39 ± 5.45 aa | 65.29 ± 8.37 | 55.19 ± 8.19 | 60.06 ± 10.27 | 58.19 ± 6.26 δ | |
| CAT | 7.41 ± 0.85 | 4.12 ± 0.99 | 6.62 ± 1.04 | 2.72 ± 0.61 a | 8.01 ± 1.24 | 5.92 ± 0.93 | 7.22 ± 1.02 | 6.12 ± 0.67 | |
Plasma corticosterone (CORT) levels were measured from tail bloods samples in all rats during the last day of AO treatment (between 6:00 p.m. and 7:00 p.m.). Oxidative stress (OS) was measured in the amygdala brain subregion, 24 h after 11 weeks of intermittent 20% v/v ethanol free access. The results are expressed as mean ± SEM (from 5 representative rats per group) of the MDA: Malondialdehyde (nmol/g of homogenate); NO: Nitrite content (μmol/g of homogenate); SOD: Superoxyde dismutase activity (µmol/min/mg of protein) and CAT: Catalase activity (µmol/min/mg of protein) in Ctrl, IEI, UMS and IEI/UMS rats with or without AO treatment. a p < 0.05, aa p < 0.01 compared with Ctrl; c p < 0.05 compared with UMS; δ p < 0.05 AO effect, according to three-way ANOVA, followed by Tukey’s test.