Fig 2. Rv3377-3378c-dependent lipids share biochemical properties consistent with a larger family of terpene nucleosides.

(A) The 254 significantly changed events in the blue box in panel Fig 1D were filtered to remove 151 recognizable isotopes and alternate adducts, yielding 104 unique molecules. These Rv3378c-dependent events restored by complementation clustered in mass and time with either 1- and N⁶-TbAd. (B) Heatmap of 15 Rv3378c-dependent events that had mass (within 10 ppm) and retention time (within 3 min) analogs across the 2 independent experimental data sets shown in Fig 1C and 1D. Rows show the ion intensity scaled separately for each experiment. Chemical modifications to TbAd consistent with the observed mass are indicated. Rows in italics were subsequently validated. Raw data for Fig 2B and 2C can be found in S1 Data.