TABLE 1.

Sequences and nucleotide positions of primers used for PCR-based site-directed mutagenesis

Primer	Sequencea (5′→3′)	Nucleotide positions
PMD	CGCAAAATACCTATGGGAGTG	1602–1622
PMU	GTTCACGGTGGTCTCCATGCG	630–650
PA617	AAAACACAG∗TTGATTTTTTGTACAATATG	921–951
PS617	AAATCAAACTGTGTTTTAGGAAACTTCCTG	939–969
PA652	TAAAGCAGGATATCCACATTGCGTGAAAG	1027–1057
PS652	GTGGATATCCTGCTTTAATGCCTTTATATG	1040–1070
PA682	AGGTTCAGATACTGTTTACTTAGAAAGGC	1117–1147
PS682	AACAGTATCTGAACCTTTACCCCGTTGCTC	1129–1159

^aMutated nucleotides are underlined.