Figure 8.

Repeated administrations of METH lead to intracellular reduction of free radicals in catecholamine A1/C1 neurons of the RVLM and CVLM. Double fluorescent analysis for TH-immunoreactivity for the catecholamine marker TH and αSyn was performed in the RVLM and CVLM of mice subjected to repeated injections of saline or METH (5 mg/kg, i.p., for 5 days) and sacrificed 60 min after the challenge carried out, on day 11. Representative images of the double immunostaining for TH and αSyn in A1/C1 within the RVLM and CVLM of mice treated with saline or METH are shown in (A,B), respectively. The respective densitometric values of DHE stain optical density (OD) are shown in (A’,B’), respectively. Values are the means ± S.E.M. * p < 0.05 (two-tailed Mann–Whitney test).