Figure 11.

Repeated administrations of METH lead to intracellular increased p-cJun expression in catecholamine A1/C1 neurons of the RVLM and CVLM. Double immunofluorescent analysis for TH and p-cJun was performed in the RVLM and CVLM of mice subjected to repeated injections of saline or METH (5 mg/kg, i.p., for 5 days) and sacrificed 60 min after the challenge, carried out on day 11. Representative images of the double immunostaining for TH and p-cJun in A1/C1 within the RVLM and CVLM of mice treated with saline or METH are shown in (A,B), respectively. The respective densitometric values (OD: Optical Density) of p-cJun intracellular immunoreactivity (ir) are shown in (A’,B’), respectively. Values are the means ± S.E.M. * p < 0.05 (two-tailed Mann–Whitney test).