Table 1.
List of primers used and corresponding biomarkers’ nucleic acid sequences.
| Name | Primer (5′→3′) | Length | GC% | Tm | Target Gene | Amplified Size (bp) | Amplified Area |
|---|---|---|---|---|---|---|---|
| PPAR-[F] PPAR-[R] |
GAGGGCACACGCTAGGAAG GGACTCGAAGTAGCGGACAG |
19 20 |
63 60 |
60 60 |
XM_006242152.4 | 72 | GAGGGCACACGCTAGGAAGGGCACACGCGTGCGACTTTCGGGGCCCCTGGAACTGTCCGCTACTTCGA GTCC |
| PPAR-[F] PPAR-[R] |
GCCGCCTCAGATTTGAAAGAA GTCCACAGAGCTGATTCCGA |
21 20 |
48 55 |
59 60 |
XM_006237009.4 | 142 | GCCGCCTCAGATTTGAAAGAAGCTGTGAACCACTAATATCCAAGGACATTTTTGAAAACAAGGACTACCCTTTACTGAAATTACCATGGTTGACACAGAGATGCCATTCTGGCCCACCAACTTCGGAATCAGCTCTGTGGAC |
| APN [F] APN [R] |
CAAGCGCTCCTGTTCCTCTT TGGGTCACCCTTAGGACCAA |
20 20 |
55 55 |
62 62.6 |
XM_039087986.1 | 228 | CAAGCGCTCCTGTTCCTCTTAATCCTGCCCAGTCATGAAGGGATTACTGCAACCGAAGGGCCAGGAGCTTTGGTCCCTCCACCCAAGGAAACTTGTGCAGGTTGGATGGCAGGCATCCCAGGATATCCTGGTCACAATGGGATACCGGGCCGTGATGGCAGAGATGGCACTCCTGGAGAGAAGGGAGAGAAGGGAGACGCAGGTGTTCTTGGTCCTAAGGGTGACCCA |
| Sirtuin 2 [F] Sirtuin 2 [R] |
GACGAGCTGACCCTTGAAG TCTCCAAGTTTGCATAGAGGC |
19 21 |
58 48 |
63 63 |
NM_001399630.1 | 148 | GACGAGCTGACCCTTGAAGGAGTGACACGCTACATGCAGAGCGAGCGCTGTCGCAGGGTCATCTGTTTGGTGGGAGCTGGAATCTCCACATCCGCAGGAATCCCTGACTTCCGCTCCCCATCCACTGGCCTCTATGCAAACTTGGAGA |
| Atp2a2 [F] Atp2a2 [R] |
TTTGGGCAGGATGAGGATG TTGTGGGAAGGTTCAACTCG |
19 20 |
53 50 |
66 65 |
NM_001110139.2 | 128 | TTTGGGCAGGATGAGGATGTGACATCAAAGGCTTTTACAGGGCGAGAATTTGATGAATTAGCCCCTCAGCCCAGAGAGACGCCTGCTTAAATGCCCGTTGTTTTGCTCGAGTTGAACCTTCCCACAA |
| Fasn [F] Fasn [R] |
20 20 |
55 55 |
64 63 |
NM_017332.2 | 214 | TCGCTCATGGGTGTGGAAGTGCGCCAGATCCTGGAACGTGAACATGATCTGGTGCTACCCATTCGTGAAGTACGGCAACTCACACTGCGGAAGCTTCAGGAAATGTCCTCCAAGGCTGGCTCAGACACTGAGTTGGCAGCCCCCAAGTCCAAGAATGATACATCCCTGAAGCAGGCCCAGCTGAATCTGAGTATCCTGCTGGTGAACCCTGA | |
| Leptin [F] Leptin [R] |
GAGACCCCTGTGTCGGTTC CTGCGTGTGTGAAATGTCATTG |
19 22 |
63 45 |
60 59 |
NM_013076.3 | 139 | GAGACCCCTGTGCCGGTTCCTGTGGCTTTGGTCCTATCTGTCCTATGTTCAAGCTGTGCCTATCCACAAAGTCCAGGATGACACCAAAACCCTCATCAAGACCATTGTCACCAGGATCAATGACATTTCACACACGCAG |
| rGapdh [F] rGapdh [R] |
TGATTCTACCCACGGCAAGT AGCATCACCCCATTTGATGT |
20 20 |
50 45 |
64 65 |
NM_017008.4 | 142 | TGACTCTACCCACGGCAAGTTCAACGGCACAGTCAAGGCTGAGAATGGGAAGCTGGTCATCAACGGGAAACCCATCACCATCTTCCAGGAGCGAGATCCCGCTAACATCAAATGGGGTGATGCT |
Notes: APN, adiponectin; F, forward; R, reverse; %, percent.