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Cell viability of HSC-4 (A) and HSC-7 (D) after treatment with various concentrations of betaine for 24 h. Proliferation of HSC-4 (B) and HSC-7 (E) at 1, 3, and 7 days after betaine treatment. Colony forming unit (CFU) analysis after 14 days of betaine treatment in HSC-4 (C) and HSC-7 (F). Bar and asterisks indicate a significant difference. Each small circle represents an individual sample. The data are shown in mean ± SE, n = 4.
