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. 2024 Sep 25;25(19):10295. doi: 10.3390/ijms251910295

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Invasion assay and quantitative analysis of the percentage of invaded cells. Representative images and quantitative analysis of invaded cells in HSC-4 after 24 h (A) and 48 h (B) of betaine exposure. Representative images and quantitative analysis of invaded cells in HSC-7 after 24 h (C) and 48 h (D) of betaine treatment. A normal culture medium was used as a control. Bar and asterisks indicate a significant difference. Each small circle represents an individual sample. The data are shown in mean ± SE, n = 4.