Figure 5.

Exo-GPX5 derived from 293T cells can deliver GPX5 protein to sperm cells and accumulate over time. (A) Exo-GPX5 and Exo-pcDNA3.1 were labeled with the fluorescent dye PKH67 and excess dye was removed by ultracentrifugation. The supernatant after centrifugation was added to sperm cells as a control group, and PKH67-labeled exosomes were co-incubated with sperm cells for 24 h (PKH67 in green, DAPI in blue); scale bar = 10 µm. (B,C) After incubating sperm with Exo-GPX5 and Exo-pcDNA3.1 for 5 days, GPX5 and HA protein expression in sperm cells was analyzed by Western blot, with α-tubulin used as the internal control (n = 3). (D) Expression levels of HA and GPX5 through densitometric analysis of Western blot bands (n = 3). Different lowercase letters indicate significant differences at p < 0.05, different uppercase letters indicate extremely significant differences at p < 0.01 and the same letters indicate no significant differences. All values are expressed as mean ± SEM (n = 3).