Figure 6.

Effects of different concentrations of Exo-GPX5 on sperm quality. (A) Sperm motility assessment (n = 4). (B) Acrosome integrity detection using FITC-PNA and DAPI double staining: (a) sperm with intact acrosomes, (b) sperm with damaged acrosomes, (c) sperm with lost acrosomes; scale bar = 20 μm. (C) Analysis of sperm acrosome integrity rate (n = 3); different lowercase letters indicate significant differences at p < 0.05, different uppercase letters indicate extremely significant differences at p < 0.01 and the same letters indicate no significant differences. All values are expressed as mean ± SEM.