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. 2024 Oct 2;25(19):10614. doi: 10.3390/ijms251910614

Figure 3.

Figure 3

Early neuronal differentiation of three HPDL patient-derived hiPS lines. (A) Schematic representation of neuronal differentiation protocol to obtain neocortical neurons, starting from human fibroblast-reprogrammed hiPS cells. (B1–B4, C1–C4, D1–D4, E1–E4) Representative confocal images with neuronal markers TUBB3 and FOXG1 in CTRL and all HPDL immature neurons. All nuclei were marked with DAPI. Scale bar: 50 μm. (F) Bar plot indicating the ratio of FOXG1 positive cells on total nuclei in CTRL and HPDL cell lines (counted cells: CTRL, N = 4951; Patient 1, N = 3658; Patient 2, N = 3404; Patient 3, N = 3991).