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. 2024 Sep 30;25(19):10572. doi: 10.3390/ijms251910572

Figure 3.

Figure 3

Effects of the synergic combination of CUR+PLD before TMZ treatment on U87 and LN18 cells. (a,c) Phase contrast microscopy images showing the morphological changes induced by 24 h CUR+PLD treatment on U87 (a) and LN18 (c) cells. The scale bars represent 100 μm. (b,d,e) WB and analysis of modifications induced by 24 h CUR+PLD treatment on expression levels of the proliferation marker c-Myc, the astroglial differentiation marker GFAP, the autophagosomal marker LC3B and the marker of caspase-dependent apoptosis PARP. Histograms in (b,d) report the densitometric analysis results for GFAP, c-Myc, and LC3B expression; values were normalized to GAPDH. Results are the mean ± SD from three independent experiments (n = 3). Significance vs. control (two-tailed Student’s t-test): * p < 0.05, ** p < 0.01; ns: not significant.