Figure 3.

Detection and verification of MAPK6-interacting proteins. (A) Mass spectrometry analysis showing MKK4, BLE1, and BIM2 were co-purified with MAPK6 in vivo. (B) Yeast two-hybrid assays indicate MAPK6 and MAPK6^sor8 interact with BIM2 and MKK4. The yeast clones were cultured on SD medium lacking Leu and Trp, and interaction detected on SD medium lacking Leu, Trp, His, and Ade and containing X-α-gal. (C) Bimolecular fluorescence complementation assays showing interactions between MAPK6–BIM2 and MAPK6–MKK4 in tobacco leaves. Scale bar, 50 μm.