Figure 2.

RNASE4 knockdown suppresses oncogenic phenotypes of GBM. A. Western blot analysis was employed to confirm RNASE4 knockdown using two RNASE4-specific shRNAs in the cell lysates and conditioned medium of DBTRG-05MG cells. ACTIN served as the internal control for cellular lysates, while Amido Black staining of total secreted proteins served as the loading control for proteins in the conditioned medium. B, C. Colony formation and proliferation assays was evaluated in DBTRG-05MG cells with or without RNASE4 knockdown (**P<0.01). D. Transwell migration and Matrigel invasion assays were performed to evaluate changes in cell motility following RNASE4 knockdown. The right panels display statistical results from each assay, with significance indicated as P<0.01. E. The sphere formation ability of RNASE4-depleted DBTRG-05MG cells, achieved using two different shRNAs, was assessed to determine the impact of RNASE4 knockdown. Scale bar, 100 μm. Statistical significance is indicated as P<0.01. F. MTS assays were conducted to evaluate the effect of RNASE4 on cellular chemoresistance to TMZ in control versus RNASE4-KD DBTRG-05MG cells over a period of 3 days. Data are presented as mean ± SEM from three independent experiments. Data are mean ± SEM of three independent experiments (**P<0.01).