Figure 1.

Zeb1 regulates the M2-like polarization of TAMs. (A) Flow chart of immunosuppressive cell dissociation. (B) Flow cytometry analysis of immunosuppressive cells in breast cancer tissues (n = 4 for both the PyMT group and the PyMT;Zeb1^cKO group). (C) Relative mRNA levels of M1- and M2-TAM markers in F4/80⁺ macrophages sorted from breast cancer tissues (n = 4 for both the PyMT group and the PyMT;Zeb1^cKO group). (D) Immunofluorescence staining for F4/80 and CD206 in breast cancer tissues (n = 5 for both the PyMT group and the PyMT;Zeb1^cKO group). (E) Relative mRNA levels of M1- and M2-TAM markers in peritoneal macrophages treated with CM from primary cancer cells (n = 5 for both the PyMT group and the PyMT;Zeb1^cKO group). (F, G) Flow cytometry analysis of CD206⁺ (F) and TGF-β1⁺ (G) cells in peritoneal macrophages treated with CM from primary cancer cells (n = 5 for both the PyMT group and the PyMT;Zeb1^cKO group). (H) Analysis of the migration of peritoneal macrophages treated with CM from primary cancer cells using the Transwell assay (n = 5 for both the PyMT group and the PyMT;Zeb1^cKO group). (I) Western blotting analysis of Zeb1 expression in Zeb1-expressing MDA-MB-231 cells. (J) Relative mRNA levels of M1- and M2-TAM markers in THP1 macrophages treated with CM from Zeb1-expressing MDA-MB-231 cells. (K, L) Flow cytometry analysis of CD206⁺ (K) and TGF-β1⁺ (L) THP1 macrophages treated with CM from Zeb1-expressing MDA-MB-231 cells. (M) Transwell assay analysis of the migration of THP1 macrophages treated with CM from Zeb1-expressing MDA-MB-231 cells. The indicated P values were calculated using two-tailed unpaired Student’s t-tests. The data are presented as the mean ± SEM in (B-H). The dots represent individual samples in (B-H). The data are representative of four (B, C), five (D-H) or three (J-M) independent experiments. Source data are provided as a source data file.