Figure 4.

Tumor cell-derived Cxcl1 is predominantly responsible for M2-TAM polarization in the TME. (A) Representative image of tumors dissected from a xenograft mouse model treated with a Cxcl1-neutralizing antibody. (B, C) The volume (B) and weight (C) of tumors generated by parental primary tumor cell injection into the mammary fat pads of C57BL/6 mice. (D) Immunofluorescence staining for F4/80 and CD206 in breast cancer tissues (n = 5 for the PyMT group, the PyMT+anti-Cxcl1 group, the PyMT;Zeb1^cKO group, and the PyMT;Zeb1^cKO+anti-Cxcl1 group). (E) Representative image of tumors dissected from xenograft mice model with or without Cxcl1-neutralizing antibody treatment. (F, G) The volume (F) and weight (G) of tumors generated by parental primary tumor cell injection into the mammary fat pads of C57BL/6 mice with or without Cxcl1-neutralizing antibody treatment. (H) Immunofluorescence staining for F4/80 and CD206 in breast cancer tissues (n = 5 for the PyMT group, the PyMT;Zeb1^cKO group, the PyMT+Cxcl1^-/- group, and the PyMT;Zeb1^cKO+Cxcl1^-/- group). The indicated P values were calculated using two-tailed unpaired Student’s t-tests. The data are presented as the means ± SEMs in (B-D) and (F-H). The dots represent individual samples in (C-D) and (G-H). The data are representative of five (A-H) independent experiments. Source data are provided as a source data file.