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. 2024 Sep 15;14(9):4378–4397. doi: 10.62347/UAIS7070

Figure 5.

Figure 5

Cxcl1 induces M2-type TAM polarization via Jak-Stat3 signaling. (A) GSEA of transcriptome data from THP1_Cxcl1 vs. THP1 cells showing enrichment of gene signatures associated with M2-like polarization and the Jak-Stat pathway in THP1_Cxcl1 cells. (B) Western blotting analysis of Stat3 and phosphorylated Stat3 (pStat3) levels in THP1 macrophages treated with rhCxcl1 in the presence of either the Cxcr2 or Stat3 inhibitor. (C) Relative mRNA levels of M2-TAM markers in THP1 macrophages treated with rhCxcl1 in the presence of either the Cxcr2 or Stat3 inhibitor. (D) Transwell migration assay of THP1 macrophages treated with rhCxcl1 in the presence of either the Cxcr2 or Stat3 inhibitor. (E, F) Flow cytometry analysis of CFSE+ cells among CD8+ (E) and CD4+ (F) T cells cocultured with peritoneal macrophages treated with rmCxcl1 in the presence of Stat3 inhibitor. (G, H) Relative mRNA levels of TNF-α and IFN-γ in CD8+ T cells cocultured with peritoneal macrophages treated with rmCxcl1 in the presence of Stat3 inhibitor. The indicated P values were calculated using two-tailed unpaired Student’s t-tests. The data are presented as the mean ± SEM in (C-H). Source data are provided as a source data file.