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. 2024 Sep 15;14(9):4337–4352. doi: 10.62347/KCPN6689

Figure 2.

Figure 2

NR4A2 regulates HuR (ELAVL1) and IDH1 in pancreatic cancer cells. A. RNA sequencing was performed in MiaPaCa2 cells (gene edited by CRISPR-Cas9) [14] and MiaPaCa2 wildtype. Volcano plot showing significant changes in gene expression in NR4A2(-/-) cells as compared to NR4A2(+/+) cells. B. Western blot analysis and RT-qPCR analysis of HuR and IDH1 after silencing of NR4A2. C. Western blot and RT-qPCR analysis of HuR and IDH1 expression after CRISPR knockout of NR4A2 in MiaPaCa2 cells. D. ChIP analysis in MiaPaCa2 for HuR and IDH1 (HuR and IDH1 promoter) bound to NR4A2 protein relative to IgG as determined by RT-qPCR. Quantitated results are means ± SE for at least 3 replicate determinations; significance, **P<0.01, ***P<0.001.