Figure 3.

NR4A2 regulated expression of HuR and IDH1 involves interactions with Sp transcription factors. (A) Schematic representation of NR4A2 binding to Sp4 and regulating gene transcription. (B) Expression of gene products after RNA silencing of Sp1, (C) Sp4 and (D) treatment with mithramycin in pancreatic cancer cell lines (MiaPaCa2 and Panc1). (E) Co-immunoprecipitation of NR4A2 and Sp4 using NR4A2 antibodies and subsequent western blot analysis as outlined in the methods. Sp4 antibodies also co-immunoprecipitated Sp4 and NR4A2 however the NR4A2 band was weak (data not shown).