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. 2024 Sep 15;14(9):4337–4352. doi: 10.62347/KCPN6689

Figure 4.

Figure 4

C-DIM12 inhibits cell proliferation and migration and downregulates expression of HuR and IDH1. (A) The structure of DIM-pPhCl (C-DIM12) and percentage of cell survival after treatment with 0-1000 μM of C-DIM12 for 24 hours. (B) MiaPaCa2 and Panc1 cells were treated with 15 μM C-DIM12 for 24 hours and apoptosis was measured by Annexin V staining (images were taken at 4× magnification). (C) Soft agar colony formation assay was determined in pancreatic cancer cells treated with 15 μM C-DIM12 for 4 weeks. (D) Cell migration images and quantification of migrated cells in a scratch assay after treatment of pancreatic cancer cells with C-DIM12 for 0 or 48 hours. (E) Western blot and (F) RT-qPCR analysis of HuR and IDH1 levels in pancreatic cancer cells after treatment with C-DIM12 for 24 hours. (G) ChIP assay results showing that C-DIM12 decreases interaction of NR4A2 with the HuR and IDH1 gene promoter (2 primer sets used for each gene). Quantitated results are means ± SE for at least 3 replicate determinations; significance, *P<0.05, **P<0.01, ***P<0.001.