Skip to main content
. 2024 Sep 15;14(9):4337–4352. doi: 10.62347/KCPN6689

Figure 5.

Figure 5

HuR regulates the expression of NR4A2. (A) Relative mRNA levels of NR4A1, NR4A2 and NR4A3 in MiaPaCa2 cells after transfection with siCTRL or siHuR. (B) Immunoprecipitation analysis in MiaPaCa2 for NR4As mRNAs bound to HuR protein relative to IgG and (C) preferential immunoprecipitation of HuR by HuR antibodies and not IgG. (D) Western blot analysis of HuR and NR4A2 expression in MiaPaCa2 and Panc1 cells transfected with siCTRL (control/non-specific) and siNR4A2. Quantitated results are means ± SE for at least 3 replicate determinations; significance, ***P<0.001.