Figure 7.

C-DIM12 inhibits tumor growth in vivo. (A) Mice were injected with MiaPaCa2 cells and were treated with vehicle and C-DIM12 (50 mg/kg) when tumors reached the size of 100 mm³. Images represents the tumor size at the end of the experiment. (B) Tumor growth curve for MiaPaCa2 xenografts in nude mice, treated with 50 mg/kg C-DIM12 or vehicle control 3 times a week and (C) body weight changes in nude mice, treated with 50 mg/kg C-DIM or vehicle control 3 times a week over a period of 4 weeks and (D) scatter plots showing tumor volumes in treated and control group determined 6 weeks after initial injection. (E) Survival was evaluated after the last injection of C-DIM12. (F) Relative HuR and IDH1 mRNA levels in tumors derived from MiaPaCa2 cell xenografts (vehicle control and C-DIM12 treated). (G) Percentage of Ki-67 positive cells in tumors from vehicle control and C-DIM12 treated mice bearing MiaPaCa2 cells xenografts and Ki-67 positive cells in tumors. (H) Percentage of caspase-3 positive cells in vehicle control and C-DIM12 treated mice and (I) relative average TUNEL-positive cells in vehicle control and C-DIM treated mice. Quantitated results are means ± SE for at least 3 replicate determinations; significance, *P<0.05, **P<0.01, ***P<0.001.