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. 2024 Sep 25;14(9):4551–4566. doi: 10.62347/XTRC3347

Figure 1.

Figure 1

IDO1 overexpression impedes cellular senescence and confers CRC cells more tolerable to 5-FU. (A) The expression of IDO1 in colorectal adenocarcinoma (COAD) was analyzed using GEPIA. (B) Immunohistochemical staining was performed to analyze IDO1 expression in intestinal tissue sections from the AOM/DSS group and control. (C, D) HCT-116 and HCT-8 cells were infected with IDO1 overexpression or control lentivirus followed by the detection of IDO1 expression via western blot (C) and qPCR analysis (D). (E) Cell viability of control cells and IDO1-overexpression cells was analyzed via MTT assay. (F) Colony formation assays were performed using HCT-116 cells with stable IDO1 overexpression in the presence or absence of 20 μM 5-FU treatment. (G) The summarized colony numbers were derived from (F). (H) SA-β-gal staining of CRC control cells and IDO1-overexpression cells in the presence of 20 μM 5-FU treatment for 48 h. (I) The quantitative results of SA-β-gal positive cells from (H). Data are presented as means ± SD. *P < 0.05; **P < 0.01.