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. 2024 Sep 25;14(9):4551–4566. doi: 10.62347/XTRC3347

Figure 2.

Figure 2

IDO1 silencing drives CRC cell senescence and blunts the sensitivity of CRC cells to 5-FU. (A, B) HCT-116 and HCT-8 cells were stably infected with two different IDO1 shRNAs or scramble shRNA (shCtrl), and knockdown efficiencies were determined by western blot (A) and qPCR analysis (B). (C) Cell viability of CRC control cells and IDO1-knockdown cells was analyzed by MTT assay. (D) Colony formation assays were performed in CRC control cells and IDO1-knockdown cells in the presence or absence of 20 μM 5-FU treatment. (E) The colony numbers were summarized from (D). (F) SA-β-gal staining of CRC control cells and IDO1-knockdown cells in the presence of 20 μM 5-FU treatment for 48 h. (G) The SA-β-gal positive cells from (F) were summarized. (H) SA-β-gal activity staining was performed in HCT-116 cells expressing shCONT or shIDO1 without 5-FU treatment, and the SA-β-gal positive cells were calculated. (I) HCT-116 cells with stable IDO1 knockdown or overexpression were exposed to various concentrations of 5-FU for 48 h and the cell viabilities were determined by MTT assay. *P < 0.05; **P < 0.01.