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. 2024 Sep 25;14(9):4551–4566. doi: 10.62347/XTRC3347

Figure 3.

Figure 3

IDO1 disables senescence and confers chemoresistance of CRC cells via attenuating IGFBP5/p53 signaling. A. The levels of p53, p27, p21, and cyclin D1 in CRC cells stably expressing IDO1 were determined by western blot. B. The levels of p53, p16, p21, and cyclin D1 were measured by qPCR analysis in HCT-116 and HCT-8 cells expressing IDO1. C. Relative mRNA expression of p53, p16, p21, and cyclin D1 was analyzed by qPCR analysis in the CRC cells with IDO1 knockdown. D. Western blot was performed to detect protein levels of p53, p27, p21, and cyclin D1 in the CRC cells with IDO1 knockdown. E. The expression of the indicated senescent-related genes was detected in HCT-116 cells with IDO1 silencing. F. Relative mRNA expression of IGFBP5 and TBX2 was analyzed by qPCR analysis in HCT-116 cells overexpressing IDO1. G. Relative mRNA expression of p53, p21, and p27 were analyzed by qPCR analysis in the IDO1-knockdown HCT-116 cells with or without IGFBP5 silencing. H. Western blot of IGFBP5 and p53 in IDO1-depleted HCT-116 cells transfected with or without siIGFBP5. I. Cell viability of IDO1-depleted HCT-116 cells with or without IGFBP5 silencing in the presence of indicated concentrations of 5-FU was measured by MTT assay. *P < 0.05; **P < 0.01.