Figure 2.

L. bicolor root components (LBR) had a significant inhibitory effect on TNBC cell proliferation. A. Half-maximal inhibitory concentration (IC₅₀) (mg/mL) values of LBR-treated cells were detected in MDA-MB-468, MDA-MB-231, and BT549 cells after treatment with LBR (0-100 μg/mL) for 48 h, respectively. B. The colony formation assay was performed after treatment with the indicated concentration of LBR for 24 h. C. Colony formation counting. D. MDA-MB-468, MDA-MB-231, and BT549 cells were stained with Annexin V/PI after LBR treatments for 24 h, and then analyzed by flow cytometry. E. Apoptosis rate in BT549 after treatment with LBR. Results are presented as mean ± standard deviation (SD), *P < 0.05 and **P < 0.01, vs. untreated group, n = 3.